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antiulbp2  (R&D Systems)


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    Structured Review

    R&D Systems antiulbp2
    Antiulbp2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antiulbp2/Human+ULBP-2%2F5%2F6+Alexa+Fluor%C2%AE+647-conjugated+Antibody/10__1158_slash_2326___6066__cir___21___0587-115-37-39
    Average 91 stars, based on 1 article reviews
    antiulbp2 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Control:

    Article Title: Soluble ligands for the NKG2D receptor are released during HIV-1 infection and impair NKG2D expression and cytotoxicity of NK cells.
    Article Snippet: In humans, the interaction of the natural killer group 2 member D (NKG2D)-activating receptor on natural killer (NK) and CD8 T cells with its major histocompatibility complex class I-related chain (MIC) and UL16 binding protein (ULBP) ligands (NKG2DLs) promotes recognition and elimination of stressed cells, such as tumor or infected cells.. Here, we investigated the capacity of HIV-1 to modulate NKG2DL expression and escape NGK2D-mediated immunosurveillance.. In CD4 T lymphocytes, both cell surface expression and release of MICA, MICB, and ULBP2 were up-regulated >2-fold by HIV-1 infection.

    Staining:

    Article Title: CD34<sup>+</sup> progenitor-derived NK cell and gemcitabine combination therapy increases killing of ovarian cancer cells in NOD/SCID/IL2Rg<sup>null</sup> mice
    Article Snippet: OC cells and HPC-NK cells were immediately stained after drug treatment, or when staining HPC-NK cells for deathinducing ligands, after stimulation with K562 for 4 h. Staining was performed after 5–10 min incubation with nanogam (total human immunoglobulins; Sanquin, 8717185830262). .. Parental OC cell lines, ASC 009, and dissociated SKOV-3-lucGFP tumor nodules were stained using viability dye ViaKrome808 (Beckman Coulter, C36628) and the following antibodies: antiULBP2/5/6-PE (R&D Systems, FAB1298P), CD261(TRAIl-R1)PE-Atto594 (Novus Biologicals, NBP2-31343PEATT594), CD112 (Nectin-2)-PE-Cy7 (Biolegend, 337414), CD262(TRAIL-R2) -APC (Biolegend, 307407), CD155(PVR)-BV421 (Biolegend, 337631), and CD95(Fas)-BV785 (Biolegend, 305645); or antiMICA/B-PE (Biolegend, 320906), anti-ULBP1-PE-Atto594 (Novus Biologicals, NBP2-11986PEATT594), anti-ULBP3-APC (R&D Systems, FAB1517A), anti-HLA-ABC-APC-Fire750 (Biolegend, 311443), and anti-HLA-E-BV421 (Biolegend, 342611). .. HPC-NK cells were stained with CD56-BV510 (Biolegend, 318340), CD253(TRAIL)-APC (Biolegend, 308210), CD178 (FasL)-PE (Biolegend, 306407), and fixable viability dye eFluor780; or CD45-BUV395 (BD Biosciences, 563792), CD56BV421 (Biolegend, 318328), CD3-BV605 (Biolegend, 300460), CD159a(NKG2A)-APC (Beckman Coulter, A60797), CD158bBV510 (BD Biosciences, 743452), CD158e1-AF700 (Biolegend, 312712), CD69-BUV737 (BD Biosciences, 612818), CD337 (NKp30)-PE-Cy5 (Beckman Coulter, A66904), CD336(NKp44)PE-Cy7 (Biolegend, 325116), CD335(NKp46)-PE-Dazzle594 (Biolegend, 331930), CD226(DNAM-1)-PE (Biolegend, 337106), CD314(NKG2D)-BV785 (Biolegend, 320830), and viability dye ViaKrome808 (Beckman Coulter, C36628).

    Article Title: Targeting WEE1/AKT Restores p53-Dependent Natural Killer–Cell Activation to Induce Immune Checkpoint Blockade Responses in “Cold” Melanoma
    Article Snippet: Cells were trypsinized, washed with PBS (Hyclone), and labeled with 7-aminoactinomycin D (7AAD; 0.25 mg, BD Pharmingen 51-68981E) and incubated for 15 minutes in the dark to exclude nonviable cells. .. Cells were washed twice with FACS buffer [2% FBS (R&D Systems) and 0.1% NaN3 (Millipore Sigma) in PBS] and stained with different NK ligand antibodies, anti-MICA-PE (FAB1300P, R&D Systems), anti-MICB-AF488 (FAB1599G, R&D Systems), anti-ULBP-1-PerCP (FAB1380C, R&D Systems), antiULBP2/5/6-AF647 (FAB1298R, R&D Systems), anti-ULBP3-AF450 (FAB1517S, R&D Systems), anti-Nec2-PE-Cy7 (clone: TX31, 337414, BioLegend) at 1:100 dilution and incubated for 30 minutes at room temperature in the dark. .. Cells werewashed twicewith FACSbuffer and all samples were immediately run on a BD LSR Fortessa flow cytometer, and the data were analyzed using FlowJo software (v10.7).

    FACS:

    Article Title: Targeting WEE1/AKT Restores p53-Dependent Natural Killer–Cell Activation to Induce Immune Checkpoint Blockade Responses in “Cold” Melanoma
    Article Snippet: Cells were trypsinized, washed with PBS (Hyclone), and labeled with 7-aminoactinomycin D (7AAD; 0.25 mg, BD Pharmingen 51-68981E) and incubated for 15 minutes in the dark to exclude nonviable cells. .. Cells were washed twice with FACS buffer [2% FBS (R&D Systems) and 0.1% NaN3 (Millipore Sigma) in PBS] and stained with different NK ligand antibodies, anti-MICA-PE (FAB1300P, R&D Systems), anti-MICB-AF488 (FAB1599G, R&D Systems), anti-ULBP-1-PerCP (FAB1380C, R&D Systems), antiULBP2/5/6-AF647 (FAB1298R, R&D Systems), anti-ULBP3-AF450 (FAB1517S, R&D Systems), anti-Nec2-PE-Cy7 (clone: TX31, 337414, BioLegend) at 1:100 dilution and incubated for 30 minutes at room temperature in the dark. .. Cells werewashed twicewith FACSbuffer and all samples were immediately run on a BD LSR Fortessa flow cytometer, and the data were analyzed using FlowJo software (v10.7).

    Incubation:

    Article Title: Targeting WEE1/AKT Restores p53-Dependent Natural Killer–Cell Activation to Induce Immune Checkpoint Blockade Responses in “Cold” Melanoma
    Article Snippet: Cells were trypsinized, washed with PBS (Hyclone), and labeled with 7-aminoactinomycin D (7AAD; 0.25 mg, BD Pharmingen 51-68981E) and incubated for 15 minutes in the dark to exclude nonviable cells. .. Cells were washed twice with FACS buffer [2% FBS (R&D Systems) and 0.1% NaN3 (Millipore Sigma) in PBS] and stained with different NK ligand antibodies, anti-MICA-PE (FAB1300P, R&D Systems), anti-MICB-AF488 (FAB1599G, R&D Systems), anti-ULBP-1-PerCP (FAB1380C, R&D Systems), antiULBP2/5/6-AF647 (FAB1298R, R&D Systems), anti-ULBP3-AF450 (FAB1517S, R&D Systems), anti-Nec2-PE-Cy7 (clone: TX31, 337414, BioLegend) at 1:100 dilution and incubated for 30 minutes at room temperature in the dark. .. Cells werewashed twicewith FACSbuffer and all samples were immediately run on a BD LSR Fortessa flow cytometer, and the data were analyzed using FlowJo software (v10.7).



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